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The Translation Laboratory |
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Available Positions for
Postdoctoral Fellows Location: School of Chemistry and Materials Science,
Hangzhou Institute for Advanced Study, University of Chinese Academy of
Sciences, 1 Sub-lane Xiangshan, Hangzhou 310024, China. For more information: |
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About TTLabTTLab stands for The
Translation Laboratory, because research groups in TTLab are
concerned with facilitating the practical application of scientific
discoveries from basic research to the development of new ways to prevent,
diagnose, and treat human diseases. TTLab can also be interpreted by Transcription-Translation
Laboratory, since transcription and translation are our two major
research directions. In the translation aspect, the FANG group is focused on
aminoacyl-tRNA synthetases. The enzymes catalyze the attachments of amino
acids to their cognate tRNAs. This is the first reaction of protein
synthesis, and it establishes the algorithm of the genetic code. The research
group investigates synthetases in their role as catalysts of aminoacylation
and how it can be intervened to treat human diseases. Synthetases in
mammalian cells are also known to have expanded functions, including
activities in signal transduction pathways, such as those for angiogenesis
and inflammation. These activities also provide opportunities for anticipated
therapeutic applications. The second direction is focused on
the Mi (MITF/TFE/USF) family of transcription factors. This family includes
several important proteins that are critical for innate immune responses,
autophagy, and tumor genesis. The WANG group
investigates the cellular regulation pathways that lead to the activation of
the Mi family. Development of drugs to target the Mi family for human disease
treatment is another goal of the WANG group. For all of these works, a
cross-disciplinary approach is used. Methods and logic of biochemistry and
molecular and cell biology are merged with genetics, x-ray crystallography,
evolutionary analysis, and high throughput drug screening. |
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Pengfei
Fang, Ph.D. Professor State Key Laboratory of Bioorganic and Natural Products
Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences https://orcid.org/0000-0002-3448-5494
Jing
Wang, Ph.D. Professor State Key Laboratory of Bioorganic and Natural Products
Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences https://orcid.org/0000-0002-7566-1212
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Joining TTLabAvailable Positions for
Postdoctoral Fellows Location: School
of Chemistry and Materials Science, Hangzhou Institute for Advanced Study,
University of Chinese Academy of Sciences, 1 Sub-lane Xiangshan, Hangzhou
310024, China. For more information: WANG group recruitment
We welcome interested
students to join our laboratory after being admitted to SIOC graduate
program. We encourage inquiries to be sent directly to Dr. Fang (fangpengfei@@sioc.ac.cn) or Dr. Wang (jwang@@sioc.ac.cn). |
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Publications>> Publications from Professor Jing Wang
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¡¤
Publications
from Professor Pengfei Fang: 46. Cheng
Y, Yi X, Zhang Y, He Q, Chen D, Cao W, Fang
P, Liu W. Oxidase Heterotetramer Completes 1-Azabicyclo[3.1.0]hexane
Formation with the Association of a Nonribosomal Peptide Synthetase. J Am Chem Soc. doi: 10.1021/jacs.2c12507.
(2023). 45. Qiao
H, Xia M, Cheng Y, Zhou J, Zheng L, Li W, Wang J, Fang P. Tyrosine-targeted covalent inhibition of a tRNA
synthetase aided by zinc ion.
Commun Biol. 6(1):107. (2023). 44. Zeng
QY, Zhang F, Zhang JH, Hei Z, Li ZH, Huang MH, Fang P, Wang ED, Sun XJ, Zhou XL. Loss of threonyl-tRNA
synthetase-like protein Tarsl2 has little impact on protein synthesis but
affects mouse development. J Biol
Chem. doi: 10.1016/j.jbc.2023.104704. (2023). 43. Lin
Y, Yu B, Fang P, Wang J.
Inhibiting autophagy before it starts. Autophagy. doi:
10.1080/15548627.2023.2197364. (2023). 42. Lin
Y, Shi Q, Yang G, Shi F, Zhou Y, Wang T, Xu P, Li P, Liu Z, Sun H, Zhao Z,
Ding K, Wang Z, Feng H, Yu B, Fang
P, Wang J. A small-molecule drug inhibits autophagy gene expression
through the central regulator TFEB. Proc Natl Acad Sci USA,
120(7):e2213670120 (2023). 41. Liu
Z, Chen K, Dai J, Xu P, Sun W, Liu W, Zhao Z, Bennett SP, Li P, Ma T, Lin Y,
Kawakami A, Yu J, Wang F, Wang C, Li M, Chase P, Hodder P, Spicer TP,
Scampavia L, Cao C, Pan L, Dong J, Chen Y, Yu B, Guo M, Fang P, Fisher DE, Wang J. A unique hyperdynamic dimer
interface permits small molecule perturbation of the melanoma oncoprotein
MITF for melanoma therapy. Cell Research, 33(1):55-70 (2023). 40. Liu
Y, Chen Y, Jiang J, Chu X, Guo Q, Zhao L, Feng F, Liu W, Zhang X, He S, Yang
P, Fang P, Sun H.
Development of highly potent and specific AKR1C3 inhibitors to restore the
chemosensitivity of drug-resistant breast cancer. European Journal of
Medicinal Chemistry, 247:115013 (2023). 39. Hei
Z, Fang P. Sequential magnesium binding facilitates lysyl-tRNA
synthetase to recognize ATP. Biochemistry and Biophysics Reports, 33:101426
(2023). 38. Yu
T, Zhang Y, Zheng WQ, Wu S, Li G, Zhang Y, Li N, Yao R, Fang P, Wang J, Zhou XL. Selective degradation of
tRNASer(AGY) is the primary driver for mitochondrial seryl-tRNA
synthetase-related disease. Nucleic Acids Research, 50(20):11755-11774 (2022). 37. Cao
X, Du X, Jiao H, An Q, Chen R, Fang
P, Wang J, Yu B. Carbohydrate-based drugs launched during 2000-2021.
Acta Pharmaceutica Sinica B, 12(10):3783-3821 (2022). 36. Zhong
B, Peng W, Du S, Chen B, Feng Y, Hu X, Lai Q, Liu S, Zhou ZW, Fang P, Wu Y, Gao F, Zhou H,
Sun L. Oridonin Inhibits SARS-CoV-2 by Targeting Its 3C-Like Protease. Small
Science, 2, 2100124 (2022). 35. Wu
S, Zheng L, Hei Z, Zhou JB, Li G, Li P, Wang J, Ali H, Zhou XL, Wang J, Fang
P. Human lysyl‑tRNA synthetase evolves a dynamic structure that
can be stabilized by forming complex. Cellular and Molecular Life Sciences,
79, 128 (2022). 34. Yang
G, Li P, Liu Z, Wu S, Zhuang C, Qiao H, Zheng L, Fang P, Lei C, Wang J. Structural basis
for the dimerization mechanism of human transcription factor E3. Biochemical
and Biophysical Research Communications, 569, 41-46 (2021). 33. Wu
S, Hei Z, Zheng L, Zhou J, Liu Z, Wang J, Fang P.
Structural analyses of a human lysyl-tRNA synthetase mutant associated with
autosomal recessive nonsyndromic hearing impairment. Biochemical and
Biophysical Research Communications, 554, 83-88 (2021). 32. Hei
Z, Wu S, Zheng L, Zhou J, Liu Z, Wang J, Fang P. Crystal
structures reveal a novel dimer of the RWD domain of human general control
nonderepressible 2. Biochemical and Biophysical Research Communications, 549,
165-170 (2021). 31. Zhou J, Huang Z, Zheng L, Hei Z,
Wang Z, Yu B, Jiang L, Wang J, Fang
P. Inhibition of Plasmodium
falciparum Lysyl-tRNA synthetase via an Anaplastic Lymphoma Kinase
Inhibitor. Nucleic Acids Research, 48, 11566-11576 (2020). 30. Zhou J, Zheng L, Hei Z, Li W,
Wang J, Yu B, Fang P.
Atomic resolution analyses of isocoumarin derivatives for inhibition of
lysyl-tRNA synthetase. ACS chemical biology, 15, 1016-1025 (2020). 29. Wang Y, Zhou JB, Zeng QY, Wu S,
Xue MQ, Fang P, Wang ED,
Zhou XL. Hearing impairment-associated KARS mutations lead to defects in
aminoacylation of both cytoplasmic and mitochondrial tRNA(Lys). Science
China. Life sciences, 63, 1227-1239 (2020). 28. Yu J, Liu Z, Liang Y, Luo F,
Zhang J, Tian C, Motzik A, Zheng M, Kang J, Zhong G, Liu C, Fang P, Guo M, Razin E, Wang
J. Second Messenger Ap4A Polymerizes Target Protein HINT1 to
Transduce Signals in Fc¦ÅRI-activated Mast Cells. Nature communications 10,
4664 (2019). 26. Hei Z, Wu S, Liu Z, Wang J, Fang P.
Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human
multi-aminoacyl-tRNA synthetase complex. The Journal of biological chemistry
294, 4775-4783 (2019). 25. Kong J, Fang P, Madoux F, Spicer TP,
Scampavia L, Kim S, et al. High-Throughput Screening for Protein Synthesis
Inhibitors Targeting Aminoacyl-tRNA Synthetases. SLAS discovery : advancing
life sciences R & D 23, 174-182 (2018). 24. Wang J, Fang P, Chase P, Tshori S,
Razin E, Spicer TP, et al. Development of an HTS-Compatible Assay for
Discovery of Melanoma-Related Microphthalmia Transcription Factor Disruptors
Using AlphaScreen Technology. SLAS discovery : advancing life sciences R
& D 22, 58-66 (2017). 23. Tao Y, Fang P, Kim S, Guo M, Young
NL, Marshall AG. Mapping the contact surfaces in the Lamin A:AIMP3 complex by
hydrogen/deuterium exchange FT-ICR mass spectrometry. PloS one 12, e0181869
(2017). 22. Fang P, Guo M. Structural
characterization of human aminoacyl-tRNA synthetases for translational and
nontranslational functions. Methods 113, 83-90 (2017). 21. Slade DJ, Fang P, Dreyton CJ, Zhang Y,
Fuhrmann J, Rempel D, et al. Protein arginine deiminase 2 binds calcium in an
ordered fashion: implications for inhibitor design. ACS chemical biology 10,
1043-1053 (2015). 20. Rachmin I, Amsalem E, Golomb E,
Beeri R, Gilon D, Fang P, et al. FHL2 switches MITF from activator to repressor
of Erbin expression during cardiac hypertrophy. International journal of
cardiology 195, 85-94 (2015). 19. Park H, Gonzalez AL, Yildirim I,
Tran T, Lohman JR, Fang P, et al. Crystallographic and
Computational Analyses of AUUCU Repeating RNA That Causes Spinocerebellar
Ataxia Type 10 (SCA10). Biochemistry 54, 3851-3859 (2015). 18. Mirando AC, Fang P, Williams TF, Baldor
LC, Howe AK, Ebert AM, et al. Aminoacyl-tRNA synthetase dependent
angiogenesis revealed by a bioengineered macrolide inhibitor. Scientific
reports 5, 13160 (2015). 17. Fang P, Yu X, Jeong SJ,
Mirando A, Chen K, Chen X, et al. Structural basis for full-spectrum
inhibition of translational functions on a tRNA synthetase. Nature
communications 6, 6402 (2015). 16. Fang P, Han H, Wang J, Chen
K, Chen X, Guo M. Structural Basis for Specific Inhibition of tRNA Synthetase
by an ATP Competitive Inhibitor. Chemistry & biology 22, 734-744
(2015). 15. Fang P, Guo M. The Nature's
Clever Trick for Making Cyclic Dinucleotide. Structure 23, 801-802
(2015). 14. Fang P, Guo M. Evolutionary
Limitation and Opportunities for Developing tRNA Synthetase Inhibitors with
5-Binding-Mode Classification. Life 5, 1703-1725 (2015). 13. Kim DG, Lee JY, Kwon NH, Fang P,
Zhang Q, Wang J, et al. Chemical inhibition of prometastatic lysyl-tRNA
synthetase-laminin receptor interaction. Nat Chem Biol 10, 29-34
(2014). 12. Zhu Y, Dai J, Zhang T, Li X, Fang P,
Wang H, et al. Structural insights into the neutralization mechanism of
monoclonal antibody 6C2 against ricin. The Journal of biological chemistry
288, 25165-25172 (2013). 11. Ofir-Birin Y, Fang P,
Bennett SP, Zhang HM, Wang J, Rachmin I, et al. Structural switch of
lysyl-tRNA synthetase between translation and transcription. Mol Cell 49,
30-42 (2013). 10. Wang J, Fang P, Schimmel P, Guo M.
Side chain independent recognition of aminoacyl adenylates by the Hint1
transcription suppressor. The journal of physical chemistry B 116, 6798-6805
(2012). 9. Jones JE, Slack JL, Fang P,
Zhang X, Subramanian V, Causey CP, et al. Synthesis and screening of a
haloacetamidine containing library to identify PAD4 selective inhibitors. ACS
chemical biology 7, 160-165 (2012). 8. Xing L, Zhu Y, Fang P,
Wang J, Zeng F, Li X, et al. Crystallization and preliminary crystallographic
studies of UbiG, an O-methyltransferase from Escherichia coli. Acta
crystallographica Section F, Structural biology and crystallization
communications 67, 727-729 (2011). 7. Wang J, Gossing M, Fang P,
Zimmermann J, Li X, von Mollard GF, et al. Epsin N-terminal homology domains
bind on opposite sides of two SNAREs. Proc Natl Acad Sci U S A 108,
12277-12282 (2011). 6. Kumar A, Park H, Fang P,
Parkesh R, Guo M, Nettles KW, et al. Myotonic dystrophy type 1 RNA crystal
structures reveal heterogeneous 1 x 1 nucleotide UU internal loop
conformations. Biochemistry 50, 9928-9935 (2011). 5. Kumar A, Fang P,
Park H, Guo M, Nettles KW, Disney MD. A crystal structure of a model of the
repeating r(CGG) transcript found in fragile X syndrome. Chembiochem : a
European journal of chemical biology 12, 2140-2142 (2011). 4. Fang P, Zhang HM, Shapiro R,
Marshall AG, Schimmel P, Yang XL, et al. Structural context for mobilization of
a human tRNA synthetase from its cytoplasmic complex. Proc Natl Acad Sci U S
A 108, 8239-8244 (2011). 3. Fang P, Li X, Wang J, Xing L,
Gao Y, Niu L, et al. Crystal structure of the protein L-isoaspartyl
methyltransferase from Escherichia coli. Cell biochemistry and biophysics 58,
163-167 (2010). 2. Fang P, Li X, Wang J, Niu L,
Teng M. Structural basis for the specificity of the GAE domain of yGGA2 for
its accessory proteins Ent3 and Ent5. Biochemistry 49, 7949-7955
(2010). 1. Fang P, Wang J, Li X, Guo M, Xing L, Cao X, et al.
Crystallization and preliminary X-ray analysis of Escherichia coli RNase G.
Acta crystallographica Section F, Structural biology and crystallization
communications 65, 586-588 (2009).
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¡¤ Publications from Professor Jing
Wang: 27. Lin
Y, Yu B, Fang P, Wang J.
Inhibiting autophagy before it starts. Autophagy. doi:
10.1080/15548627.2023.2197364. (2023). 26. Lin
Y, Shi Q, Yang G, Shi F, Zhou Y, Wang T, Xu P, Li P, Liu Z, Sun H, Zhao Z,
Ding K, Wang Z, Feng H, Yu B, Fang P, Wang
J. A small-molecule drug inhibits autophagy gene expression through
the central regulator TFEB. Proc Natl Acad Sci USA, 120(7):e2213670120
(2023). 25. Qiao
H, Xia M, Cheng Y, Zhou J, Zheng L, Li W, Wang J, Fang P. Tyrosine-targeted covalent inhibition of a
tRNA synthetase aided by zinc ion.
Commun Biol. 6(1):107. (2023). 24. Liu
Z, Chen K, Dai J, Xu P, Sun W, Liu W, Zhao Z, Bennett SP, Li P, Ma T, Lin Y,
Kawakami A, Yu J, Wang F, Wang C, Li M, Chase P, Hodder P, Spicer TP,
Scampavia L, Cao C, Pan L, Dong J, Chen Y, Yu B, Guo M, Fang P, Fisher DE, Wang J. A unique hyperdynamic
dimer interface permits small molecule perturbation of the melanoma
oncoprotein MITF for melanoma therapy. Cell Research, 33(1):55-70 (2023). 23. Hou
W, Wang M, Wang J, Cao X, Xu
P, Yu B. Collective Synthesis and Cytotoxicities of Wentilactone Type
Norditerpenoid Dilactones. Asian Journal of Organic Chemistry, e202200646
(2022). 22. Cao
X, Du X, Jiao H, An Q, Chen R, Fang P, Wang
J, Yu B. Carbohydrate-based drugs launched during 2000-2021. Acta
Pharmaceutica Sinica B, 12(10):3783-3821 (2022). 21. Wu
S, Zheng L, Hei Z, Zhou JB, Li G, Li P, Wang J, Ali H, Zhou XL, Wang J, Fang P. Human lysyl‑tRNA synthetase
evolves a dynamic structure that can be stabilized by forming complex. Cellular
and Molecular Life Sciences, 79, 128 (2022). 20. Yang
Q, Wang W, Lin Y, Lin Y, Tang Z, Wang
J, Tao J, Tang W, Liu W.
Characterization of a carboxyl methyltransferase in Fusarium graminearum provides insights into the biosynthesis of
fusarin A. Organic & Biomolecular Chemistry, 19, 6638-6643 (2021). 19. Yang
G, Li P, Liu Z, Wu S, Zhuang C, Qiao H, Zheng L, Fang P, Lei C, Wang J.
Structural basis for the dimerization mechanism of human transcription factor
E3. Biochemical and Biophysical Research Communications, 569, 41-46 (2021). 18. Ye
F, Zhao J, Xu P, Liu X, Yu J, Shangguan W, Liu J, Luo X, Li C, Ying T, Wang J, Yu B, Wang P.
Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding
Domain Reveals Different Binding Profiles of Monoclonal Antibodies.
Angewandte Chemie International Edition. 60, 12904-12910 (2021). 17. Wu
S, Hei Z, Zheng L, Zhou J, Liu Z, Wang
J, Fang P.
Structural analyses of a human lysyl-tRNA synthetase mutant associated with
autosomal recessive nonsyndromic hearing impairment. Biochemical and
Biophysical Research Communications, 554, 83-88 (2021). 16. Hei Z, Wu S, Zheng L, Zhou J,
Liu Z, Wang J, Fang P. Crystal structures reveal a
novel dimer of the RWD domain of human general control nonderepressible 2. Biochemical
and Biophysical Research Communications, 549, 165-170 (2021). 15. Zhou J, Huang Z, Zheng L, Hei Z,
Wang Z, Yu B, Jiang L, Wang J,
Fang P. Inhibition of Plasmodium
falciparum Lysyl-tRNA synthetase via an Anaplastic Lymphoma Kinase Inhibitor.
Nucleic Acids Research, 48, 11566-11576 (2020). 14. Zhou J, Zheng L, Hei Z, Li W, Wang J, Yu B, Fang P. Atomic
resolution analyses of isocoumarin derivatives for inhibition of lysyl-tRNA
synthetase. ACS chemical biology, 15, 1016-1025 (2020). 13. Yu J, Liu Z, Liang Y, Luo F, Zhang J, Tian C, Motzik A, Zheng M, Kang J, Zhong G, Liu C, Fang P, Guo M, Razin E, Wang J. Second Messenger Ap4A Polymerizes Target Protein HINT1 to Transduce Signals in Fc¦ÅRI-activated Mast Cells. Nature communications 10, 4664 (2019). 12. Hei Z, Wu S, Liu Z, Wang J, Fang P. Retractile
lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA
synthetase complex. The Journal of biological chemistry 294, 4775-4783
(2019). 11. Wang J, Fang P, Chase P, Tshori S, Razin E, Spicer TP, et
al. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related
Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology.
SLAS discovery : advancing life sciences R & D 22, 58-66 (2017). 10. Motzik A, Amir E, Erlich T, Wang J, Kim BG, Han JM, et al.
Post-translational modification of HINT1 mediates activation of MITF
transcriptional activity in human melanoma cells. Oncogene 36, 4732-4738
(2017). 9. Fang P, Han H, Wang J, Chen K, Chen X, Guo M. Structural Basis for
Specific Inhibition of tRNA Synthetase by an ATP Competitive Inhibitor.
Chemistry & biology 22, 734-744 (2015). 8. Kim DG, Lee JY, Kwon NH, Fang P, Zhang Q, Wang J, et al. Chemical
inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor
interaction. Nat Chem Biol 10, 29-34 (2014). 7. Ofir-Birin Y, Fang P, Bennett SP, Zhang
HM, Wang J, Rachmin I, et
al. Structural switch of lysyl-tRNA synthetase between translation and
transcription. Mol Cell 49, 30-42 (2013). 6. Wang
J, Fang P, Schimmel P, Guo M. Side chain independent recognition of
aminoacyl adenylates by the Hint1 transcription suppressor. The journal of
physical chemistry B 116, 6798-6805 (2012). 5. Xing L, Zhu Y, Fang P, Wang J, Zeng F, Li X, et al.
Crystallization and preliminary crystallographic studies of UbiG, an
O-methyltransferase from Escherichia coli. Acta crystallographica Section F,
Structural biology and crystallization communications 67, 727-729
(2011). 4. Wang
J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, et al.
Epsin N-terminal homology domains bind on opposite sides of two SNAREs. Proc
Natl Acad Sci U S A 108, 12277-12282 (2011). 3. Fang P, Li X, Wang J, Xing L, Gao Y, Niu L, et al. Crystal structure of
the protein L-isoaspartyl methyltransferase from Escherichia coli. Cell
biochemistry and biophysics 58, 163-167 (2010). 2. Fang P, Li X, Wang J, Niu L, Teng M. Structural basis for the
specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and
Ent5. Biochemistry 49, 7949-7955 (2010). 1. Fang P, Wang J, Li X, Guo M, Xing L,
Cao X, et al. Crystallization and preliminary X-ray analysis of Escherichia
coli RNase G. Acta crystallographica Section F, Structural biology and
crystallization communications 65, 586-588 (2009). |
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Lab MembersCurrent Lab Members
Former Lab Members
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Shipping: 2#416 China Li Zheng, Ph.D., Lab Manager Email:
lizheng2016y@@sioc.ac.cn |
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